Diagnostic Parasitology - On demand diagnostic tests

Ixodes holocyclus

Ixodes holocyclus

On demand parasitological examinations and development usually include molecular methods such as PCR and immunodiagnostic tests.

The laboratory is equipped to adopt and develop published methods or design new based on the needs of the clients. Using these activities the laboratory participates in field investigations leading to better understanding of parasite epidemiology.

Our recent extension activities include but are not limited to:
  • Australia wide flea identification and genotyping using COII PCR in collaboration with Merial Australia
    - Slapeta J, King J, McDonell D, Malik R, Homer D, Hannan P, Emery D. (2011). The cat flea (Ctenocephalides f. felis) is the dominant flea on domestic dogs and cats in Australian veterinary practices. Vet Parasitol. 180(3-4):383-8.
  • Identification of new species of brain parasite using ITS PCR in collaboration with University of Sydney Wildlife Center and Taronga ZOO
    - Hartigan A, Sangster C, Rose K, Phalen DN, Slapeta J. (2012). Myxozoan parasite in brain of critically endangered frog. Emerg Infect Dis. 18(4):693-5.
  • Adoption of direct fluorescent test to rapidly diagnose fish cryptosporidiosis in collaboration with the Future Fisheries
    - Barugahare R, Dennis MM, Becker JA, Slapeta J. (2011) Detection of Cryptosporidium molnari oocysts from fish by fluorescent-antibody staining assays for Cryptosporidium spp. affecting humans. Appl Environ Microbiol. 77(5):1878-80.
  • Multilocus PCR identification of Neospora caninum in collaboration with Taronga ZOO
    - Sangster C, Bryant B, Campbell-Ward M, King JS, Slapeta J. (2010) Neosporosis in an aborted southern white rhinoceros (Ceratotherium simum simum) fetus. J Zoo Wildl Med. 41(4):725-8.
  • Identification of distinct Tritrichomonas foetus genotype using ITS2 PCR
    - Slapeta J, Craig S, McDonell D, Emery D. (2010) Tritrichomonas foetus from domestic cats and cattle are genetically distinct. Exp Parasitol. 126(2):209-13.
  • PCR identification of a blood parasite in collaboration with Symbion Vetnostics
    - Zhu BY, Hartigan A, Reppas G, Higgins DP, Canfield PJ, Slapeta J. (2009) Looks can deceive: molecular identity of an intraerythrocytic apicomplexan parasite in Australian gliders. Vet Parasitol. 159(2):105-11.
Turnaround time

Turnaround time of routine test is usually 24 hours of sample receipt.

Turnaround time for ‘on demand’ diagnostics varies and is usually discussed with the client to meet their needs and capability of the laboratory.